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研究生中文姓名:吴惟寧
研究生英文姓名:Goh, Wei Ning
中文論文名稱:以黏度法及高效能斥濾層析法測定幾丁質之分子量
英文論文名稱:Determination of chitin molecular weight by capillary viscometer and high performance size exclusion chromatography
指導教授姓名:蔡敏郎
口試委員中文姓名:教授︰蔡敏郎
教授︰糜福龍
教授︰董崇民
助理教授︰王上達
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:食品科學系
學號:10832069
請選擇論文為:學術型
畢業年度:111
畢業學年度:111
學期:
語文別:中文
論文頁數:51
中文關鍵詞:幾丁質LiCl/DMAc氫氧化鈉/尿素氫氧化鈉/單寧酸重量平均分子量Mark-Houwink方程式
英文關鍵字:ChitinLiCl/DMAcNaOH/UreaNaOH/Tannic acidweight average molecular weightMark-Houwink equation
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摘要
Abstract
目錄
表次
圖次
1. 前言
2. 文獻回顧
2.1. 幾丁質
2.1.1. 幾丁質的發現
2.1.2. 幾丁質結構
2.1.3. 幾丁質製備
2.1.4. 幾丁質的特性及應用
2.2. 幾丁質之溶解
2.2.1. 離子溶劑
2.2.2. CaCl2/MeOH溶劑
2.2.3. 深共熔溶劑
2.2.4. LiCl/DMAc溶劑
2.2.5. NaOH/Urea溶劑
2.2.6. NaOH/Tannic Acid溶劑
2.3. 測定幾丁質分子量
2.3.1. 黏度法
2.3.2. 高效能斥濾層析法
2.3.3. 靜態光散射法
3. 實驗架構
4. 材料與儀器
4.1. 實驗材料
4.2. 藥品
4.3. 儀器設備
5. 實驗方法
5.1. 原料處理
5.1.1. 原料的前處理
5.1.2. 幾丁質的製備
5.1.3. 市售幾丁質前處理
5.2. 幾丁質樣品之製備
5.2.1. 製備不同分子量之市售α-和β-幾丁質
5.3. 幾丁質分子量之測定
5.3.1. HPSEC的應用
5.3.1.1. 移動相的配製
5.3.1.2. HPSEC配製樣品條件測試
5.3.1.3. 不同降解時間的α-和β-幾丁質分子量的測定
5.4. 還原黏度及内生性黏度之測定
5.4.1. 製備樣品
5.4.1.1. 製備5% LiCl/DMAc的幾丁質溶液
5.4.1.2. 製備8% NaOH/4% Urea的幾丁質溶液
5.4.1.3. 製備10% NaOH/0.3% Tannic acid的幾丁質溶液
5.4.2. 還原黏度及内生性黏度之測定
5.4.3. Mark-Houwink方程式黏度常數的計算
5.5. 統計分析
6. 結果與討論
6.1. 配製HPSEC樣品之條件
6.2. 不同降解時間之α-和β-幾丁質
6.3. 在LiCl/DMAc溶液α-幾丁質之内生性黏度及MH方程式黏度常數
6.4. NaOH/Urea溶液α-幾丁質之内生性黏度及其MH方程式黏度常數
6.5. NaOH/Tannic acid溶液α-幾丁質之内生性黏度及其MH方程式黏度常數
6.6. 不同溶劑系統對於内生性黏度的影響
6.7. 不同溫度對於對於内生性黏度的影響
6.8. 在三種溶劑系統中α-幾丁質的構型
6.9. 溶劑系統的配製時間對於内生性黏度的影響
7. 結論
8. 參考文獻
9. 表
10. 圖
11. 附圖
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