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研究生中文姓名:邱昭碩
研究生英文姓名:Chiu, Chao-Shuo
中文論文名稱:細胞黏附蛋白與紅血球發育
英文論文名稱:cell adhesion molecule and erythrocyte development
指導教授姓名:胡清華
口試委員中文姓名:教授︰管永恕
教授︰黃聲蘋
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:生命科學暨生物科技學系
學號:1043b023
請選擇論文為:學術型
畢業年度:106
畢業學年度:105
學期:
語文別:中文
論文頁數:33
中文關鍵詞:血球生成低氧誘發蛋白造血微環境細胞黏附蛋白斑馬魚
英文關鍵字:Hematopoietichypoxia-inducible factorhematopoietic microenvironmentcell adhesion moleculezebrafish
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目錄
摘要 I
Abstract III
前言 1
一、紅血球生成 1
二、低氧誘發蛋白 1
三、哺乳動物中的造血微環境 2
四、造血微環境中的細胞黏附分子與血管生成激素細胞訊息 3
五、實驗動機與目的 4
材料與方法 6
實驗材料 6
一、實驗生物 6
二、菌種 6
三、質體 6
四、化學藥品與實驗儀器 6
五、實驗藥品 7
實驗方法 8
(一)斑馬魚的飼養與胚胎的收集 8
(二)顯微注射斑馬魚胚胎(Microinjection) 9
(三)全覆式定位雜何反應染色(In situ Hybridization) 9
(四)即時定量反轉錄聚合酵素連鎖反應(Qunantitative RT-PCR): 10
(五)血球染色 11
(六)質體DNA抽取 11
(七)DNA之ligation 12
(八)質體轉型(Transformation) 12
(九)聚合酶連鎖反應(PCR) 12
(十) Hypoxyprobe 12
實驗結果 13
一、 胚胎發育時期造血區域的缺氧情況 13
二、 胚胎發育時期造血區域HIF1α與HIF2α蛋白質的表現 13
三、 胚胎發育時期細胞黏附分子vcam1、pecam1與血管生成素細胞受器tek基因的表現 14
四、 PECAM1和TEK與紅血球生成的關聯 14
五、HIF1α與HIF2α對tek與pecam1基因表現的影響 15
六、tek與pecam1對血管新生的影響 15
討論 16
圖表 19
參考文獻 31

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