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研究生中文姓名:程煥文
研究生英文姓名:Cheng, Huan-Wen
中文論文名稱:台灣西部沿海野生及養殖黑棘鯛(Acanthopagrus schlegelii)之生殖生物學比較
英文論文名稱:Comparison of reproductive biology of black seabream Acanthopagrus schlegelii obtaining from coastal waters off western Taiwan and aquaculture
指導教授姓名:劉秉忠
李國誥
口試委員中文姓名:助理教授︰王世斌
教授︰秦宗顯
業界委員︰黃世鈴
教授︰劉秉忠
教授︰李國誥
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學號:10433034
請選擇論文為:學術型
畢業年度:106
畢業學年度:105
學期:
語文別:中文
論文頁數:75
中文關鍵詞:黑棘鯛生殖生物學養殖與野生相比孕卵數
英文關鍵字:Acanthopagrus schlegeliireproductive biologycomparison of wild and cultured speciesfecundity
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本研究探討及比較台灣西部海域野生與養殖黑棘鯛 (Acanthopagrus schlegelii) 之生殖生物學特性,野生魚採樣期間自 2015 年 12 月至 2016 年 12 月,按月由澎湖、雲林、苗栗當地漁民依船釣、底拖網等方式捕獲,共計採得 374 尾,含雄魚 145 尾、雌魚 183 尾、雌雄同體之樣本 20 尾,以及無法分辨雌雄隻個體 26 尾;養殖魚自 2015 年 4 月由 5 公分體長開始蓄養於國立臺灣海洋大學研究生實驗溫室,採樣其間自 2016 年 1 月至 2017 年 4 月,按月隨機捕撈以 20 隻為原則進行樣本蒐集,共計採得346尾,含雄魚 219 尾、雌魚 99 尾、雌雄同體之樣本 22 尾,無法分辨雌雄之個體 6 尾。卵細胞之成熟過程依組織學方法分成八個階段,精細胞分成四個階段,而生殖腺的成熟過程可分為七個階段。以肉眼觀察生殖腺外觀、組織切片、生殖腺指數等種方法,判斷野生與養殖黑棘鯛之成熟及生殖週期,結果顯示,野生與養殖生殖期相同為 1 到 4 月,各項指數也大致相同,但在肥滿度上養殖黑棘鯛異於野生黑棘鯛,整體趨勢沒有明顯高峰值,因養殖環境與野生環境不同所致;而野生成熟體長雌魚為 25.5 cm 雄魚為 23.1 cm,養殖雌魚之性成熟體長為 26.3 cm 雄魚為 14 cm,可能因養殖密度之關係使魚體成長受限以及採樣關係,導致雄魚性成熟體長較低。野生黑棘鯛平均孕卵數 (X ± SD) 為 1,445,121 ± 714,383 粒卵,平均單次產卵數為 (X ± SD) 為1,258,034 ± 740,087 粒卵,平均相對孕卵數 (X ± SD) 為 1079 ± 441 粒卵/克,平均相對單次產卵數 (X ± SD) 為 925 ± 477 粒卵/克。養殖黑棘鯛平均孕卵數 (X ± SD) 為 479,268 ± 307,164 粒卵,平均單次產卵數為 (X ± SD)為242,783 ± 224,271粒卵,平均相對孕卵數 (X ± SD) 為 1014 ± 571 粒卵/克,平均相對單次產卵數 (X ± SD) 為 495 ± 411 粒卵/克。將養殖與野生較相近的卵巢重相比孕卵數,養殖有較多的趨勢。
This study investigated compared the reproductive biology of black sea bream, Acanthopagrus schlegelii , obtaining from the coastal water off western Taiwan and aquaculture . Wild fish were collected from local fisherman between December 2015 to December 2016, a total of 374 specimens (145 males, 183 females, 20 hermaphrodite and 26 unknown) of A. schlegelii were sampled by bottom trawler and boat fishing in Penghu, Yunlin, Miaoli conties. Farmed fish were collected between January 2016 to April 2017, a total of 346 specimens (219 males, 99 females and 22hermaphrodite 6 unknown) of A. schlegelii were sampled in National Taiwan Ocean University Graduate Laboratory Greenhouse. By means of histological examination, the oocyte development could be clearly categorized into eight stages while the gonad development could be determined in seven stages. The results showed that wild and farmed fish shared same spawning season extending from January to April based on the macroscopic appearance, histological examination of ovary, estimation of gonadosomatic index and monthly variation of percentage proportion of gonad development. However, the environmental factor of the farmed fish might be different from the wild fish. The overall trend of farmed fish exhibited no significant peak. The sized of wild fish at 50% maturity were estimated 25.5 mm FL and 23.1 mm FL for the female and male, respectively. The sizes of farmed fish at 50% maturity were estimated 26.3 mm FL and 14 mm FL for the female and male, respectively. The relatively low male sexual maturity might be due to the culture density, limited environment and possible sampling errors resulting in male sexual maturity. Mean fecundity (X ± SD), mean batch fecundity (X ± SD), relative fecundity (X ± SD) and relative batch fecundity (X ± SD) of wild fidh were estimated as 1,445,121 ± 714,383 and 1,258,034 ± 740,087 and 1079 ± 441 and 925 ± 477 (eggs/g), respectively, while thse of farmed fish were estimated as 479,268 ± 307,164 and 242,783± 224,271 and 1014 ± 571 and 495 ± 411(eggs/g), respectively. The farmed fish contained higher number of eggs than sild fish with similar ovary weight.
摘要 i
Abstract ii
圖目錄 vii
表目錄 ix
第一章 前言 1
第二章 文獻整理 3
2.1.黑棘鯛簡介 3
2.2.漁業慨況 4
2.3.放流概況 4
2.4.黑棘鯛相關研究 4
第三章 材料方法 7
3.1.樣本之收集 7
3.2.樣品魚之處理及量測 7
3.3.卵巢部位的選擇 7
3.4.組織切片之處理流程 8
3.5.資料分析 9
3.5.1.尾叉長與體重關係 9
3.5.2.性比 9
3.5.3.生殖腺指數 (Gonadosomatic index, GSI) 10
3.5.4.肝臟指數(Hepatosomatic index ; HSI) 10
3.5.5.肥滿度(Condition factor ; CF) 10
3.5.6. 50%性成熟體長之估測 10
3.5.7.孕卵數(Fecundity)、單次產卵數(Batch fecundity)、相對孕卵數(Relative Fecundity)及相對單次產卵數(Relative Batch fecundity)之計算 11
第四章 結果 12
4.1.野生黑鯛樣本之收集 12
4.2.尾叉長與體重之回歸關係 12
4.3.性比 13
4.3.1.性比月別變化 13
4.3.2.性比別體長變化 13
4.4.生殖腺組織切片觀察 14
4.4.1.卵細胞 14
4.4.2.精細胞 15
4.5.生殖腺成熟階段劃分 16
4.5.1.卵巢 16
4.5.2.精巢 16
4.5.3.雌雄同體 17
4.6.生殖期判定與能量配置 17
4.6.1生殖腺指數 (Gonadosomatic Index,GSI) 17
4.6.2.肥滿度指數 (Condition Factor,CF) 18
4.6.3.肝臟指數 (Hepatosomatic Index,HSI) 18
4.6.4.黑棘鯛生殖腺群成熟率月別頻度分佈情形 19
4.7.繁殖潛力 19
4.7.1.卵巢部位的選擇 19
4.7.2.孕卵數 20
4.7.3.單次產卵數 21
4.7.4.相對孕卵數 22
4.7.5.相對單次產卵數 22
4.8.50%性成熟體長之估計 23
4.8.1.雌魚 23
4.8.2.雄魚 23
第五章 討論 24
5.1.樣本採集 24
5.2性比討論 24
5.3產卵形態 26
5.4生殖週期及能量配置討論 26
5.4.1.生殖腺指數(Gonadosomatic Index,GSI) 27
5.4.2.肥滿度 (Condition Factor,CF) 27
5.4.3.肝臟指數 (Hepatosomatic Index,HSI) 28
5.5.繁殖潛力討論 28
5.6.50%性成熟體長 29
第六章 結論 30
第七章 參考文獻 31
圖 35
表 65

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